Tips mencampurkan bahan-bahan PCR pada tube

Ada beberapa hal yang perlu diperhatikan dalam mencampurkan bahan-bahan PCR, antara lain sebagai berikut:

1. Semua bahan harus diletakan secara terpisah pada dinding tube, kecuali akuades. Hal ini dilakukan untuk menghindari pencampuran bahan sebelum kondisi PCR dioptimasi/ diatur.

2. Bahan-bahan yang terdiri dari DNA template, MgCl2, Taq buffer, dNTP’s, dan Taq polimerase dapat dicampurkan dalam dinding tube selama preparasi bahan PCR.

3. Forward primer dan reverse primer harus diletakan agak berjauhan.

4. Akuades dapat diletakan di dasar tube.

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2 Komentar

  1. are you serious that it will be good at master mix preparation? coz, i just hear about it right now? and have you ever sucsess before? just for note, i am a biology student at university of indonesia, and now i have a research in genenetic molecular in EIJKMAN institute.

  2. Yes, no doubt for it. It is based on my experience when i was doing my field trip in Center of Biotecnology, LIPI Cibinong about 9 months ago. One of the labourant told me to try this tips. Well, that’s what i can tell you at this time. It would be better if you confirme it to the professional one.
    Btw, are you serious that you are allowed to have a research in Eijkman institute? Wow..that’s so cool. I’ve tried it, but i didn’t get the same chance as you.


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